Cell Surface Display of Thermomyces lanuginosus Lipase in Pichia pastoris
نویسندگان
چکیده
منابع مشابه
Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris.
Highly efficient production of a Thermomyces lanuginosus IOC-4145 beta-1,4-xylanase was achieved in Pichia pastoris under the control of the AOX1 promoter. P. pastoris colonies expressing recombinant xylanase were selected by enzymatic activity plate assay, and their ability to secrete high levels of the enzyme was evaluated in small-scale cultures. Furthermore, an optimization of enzyme produc...
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Use of biodiesel as an alternative to non-renewable sources of energy has become an attractive option in recent years. The enzymatic synthesis of biodiesel by transesterification of fats/oils with an alcohol is a much more sustainable route than the chemical method. However, cost effectiveness of the enzymatic route is a major barrier in its commercialization. In this work, a high activity bioc...
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BACKGROUND For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized enzymes. RESULTS Two Pichia pastoris cell surface display vectors based on the flocculation functional domain of FLO with its own secretion signal...
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Enzymatic catalysis is considered to be among the most environmental friendly processes for the synthesis of fine chemicals. In this study, lipase from Thermomyces lanuginosus (Lecitase Ultra™) was used to catalyze the synthesis of flavor esters, i.e., methyl butanoate and methyl benzoate by esterification of the acids with methanol in a microfluidic system. Maximum reaction rates of 195 and 11...
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Background Lipases are powerful tools for the syntheses of structured lipids (SL) which are triacylglycerols (TAG) having particular fatty acid residues at specific positions. The production of TAG with medium chain fatty acids (MCFA), in positions sn-1 and sn-3, and a long-chain fatty acid (LCFA), in the internal position, has recently increased due to its nutritional interest in applications ...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2020
ISSN: 2296-4185
DOI: 10.3389/fbioe.2020.544058